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Western blot analysis of extracts of various cell lines, using CBX4 antibody (22-061) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human lung cancer using CBX4 antibody (22-061) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CBX4 antibody (22-061). Western blot was performed from the immunoprecipitate using CBX4 antibody (22-061) at a dilition of 1:500.____Detection: ECL Enhanced Kit._Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using CBX4 antibody (22-061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

CBX4 Antibody

CATALOG NUMBER: 22-061

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
Mouse liver
Predicted Molecular Weight:
Observed: 85kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CBX4
Additional Names:
CBX4 Antibody: PC2, NBP16, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Pc2
Ncbi Gene Id Number:
8535
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-061

List Size:
50 uL

List Price:

$309.00

Out of stock

Datasheet
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