Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of extracts of various cell lines, using CCAR1 antibody (15-094) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using CCAR1 antibody (15-094). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CCAR1 antibody (15-094). Western blot was performed from the immunoprecipitate using CCAR1 antibody (15-094) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using CCAR1 antibody (15-094) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 5s.

CCAR1 Antibody

CATALOG NUMBER: 15-094

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
HepG2
Positive Control 4:
A-549
Positive Control 5:
A-431
Predicted Molecular Weight:
Observed: 155kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CCAR1
Additional Names:
CCAR1, cell-cycle and apoptosis regulatory protein 1, OTTHUMP00000019709, RP11-437A18.1, MGC44628, CARP1, CARP-1, cell division cycle and apoptosis regulator 1, CCAR1, DIS
Ncbi Gene Id Number:
55749
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-094

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369