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42-273 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-273 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CCAR1 Antibody

CATALOG NUMBER: 42-273

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: RYCKERPSKDKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 120kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 132kDa according to Mouse NP_080477.1 and Rat NP_001102005.1). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CCAR1
Additional Names:
CCAR1, cell division cycle and apoptosis regulator 1, CARP-1, CARP1, MGC44628, RP11-437A18.1, OTTHUMP00000019709, cell-cycle and apoptosis regulatory protein 1
Protein Accession Number:
NP_060707.2
Protein Gi Number:
46852388
Ncbi Gene Id Number:
55749
Background Reference 1:
Kim JH, Yang CK, Heo K, Roeder RG, An W, Stallcup MR, CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes. Molecular cell 2008 Aug 31 (4): 510-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-273

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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