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Western blot analysis of extracts of various cell lines, using CCAR2 antibody (23-973) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Western blot analysis of extracts of various cell lines, using CCAR2 antibody (23-973) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

CCAR2 Antibody [2E2A8]

CATALOG NUMBER: 23-973

Clonality:
Monoclonal
Tested Applications:
ICC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant protein of human CCAR2
Conjugate:
Unconjugated
Tested Applications:
ICC, IP, WB
Application Note:
WB: 1:200 - 1:1000
ICC: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
COS-7
Positive Control 2:
Jurkat
Positive Control 3:
A-549
Positive Control 4:
HeLa
Positive Control 5:
SH-SY5Y
Positive Control 6:
LO2
Predicted Molecular Weight:
Observed: 140kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CCAR2
Additional Names:
CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein
Ncbi Gene Id Number:
57805
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-973

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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