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43-639 (0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-639 (2ug/ml) staining of NIH3T3 nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-639 (0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CCAR2 / DBC1 Antibody

CATALOG NUMBER: 43-639

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ESHNRFSATEVTNK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol. 2013 Dec;33(24):4960-70. PMID: 24126058). Recommended concentration: 0.5-2ug/ml.
Predicted Molecular Weight:
Approx 140kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CCAR2 / DBC1
Additional Names:
CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein
Protein Accession Number:
NP_066997.3
Protein Gi Number:
24432106
Ncbi Gene Id Number:
57805
Background Reference 1:
Kim SH, Kim JH, Yu EJ, Lee KW, Park CK.The overexpression of DBC1 in esophageal squamous cell carcinoma correlates with poor prognosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-639

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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