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Western blot analysis of extracts of various cell lines, using CCBL1 Antibody (22-298) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using CCBL1 antibody (22-298) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CCBL1 antibody (22-298) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CCBL1 antibody (22-298). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CCBL1 Antibody (22-298) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CCBL1 Antibody

CATALOG NUMBER: 22-298

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
Raji
Positive Control 2:
HeLa
Positive Control 3:
SW620
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CCBL1
Additional Names:
CCBL1, cysteine conjugate-beta lyase, glutamine transaminase K, kyneurenine aminotransferase, GTK, KATI, MGC29624, cytoplasmic cysteine conjugate-beta lyase, glutamine-phenylpyruvate aminotransferase, kynurenine aminotransferase I, KAT1
Ncbi Gene Id Number:
883
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-298

List Size:
100 uL

List Price:

$436.00

Datasheet
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