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Western blot analysis in A549 cell line lysates (35ug/lane).
CCDC99 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in A549 cell line lysates (35ug/lane).

CCDC99 Antibody

CATALOG NUMBER: 57-083

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459-488 amino acids from the C-terminal region of human CCDC99.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
70 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPDL1
Additional Names:
Protein Spindly {ECO:0000255, HAMAP-Rule:MF_03041}, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99 {ECO:0000255, HAMAP-Rule:MF_03041}, Rhabdomyosarcoma antigen MU-RMS-404A, Spindle apparatus coiled-coil domain-containing protein 1 {ECO:0000255, HAMAP-Rule:MF_03041}, SPDL1 {ECO:0000255, HAMAP-Rule:MF_03041}
Protein Accession Number:
Q96EA4
Protein Gi Number:
296452913
Ncbi Gene Id Number:
54908
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Background Reference 1:
Barisic, M., et al. Mol. Biol. Cell 21(12):1968-1981(2010)
Background Reference 2:
Gassmann, R., et al. Genes Dev. 24(9):957-971(2010)
Background Reference 3:
Chan, Y.W., et al. J. Cell Biol. 185(5):859-874(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-083

List Size:
400 ul

List Price:

$495.00

Datasheet
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