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45-378 (1ug/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-378 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-378 (6ug/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
45-378 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.
45-378 (1ug/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CCKBR Antibody

CATALOG NUMBER: 45-378

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-FDGDSDSDSQSRVRNQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in Mouse Pancreas and Rat Stomach lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1 and 49.0kDa according to Rat NP_037297.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml. In paraffin embedded Human Pancreas expression of CCKBR is detected in islet cells, and not in surrounding acinar cells. Recommended concentration: 6ug/ml.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CCKBR
Additional Names:
CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrincholecystokinin brain receptor, CCK2R
Protein Accession Number:
NP_795344.1
Protein Gi Number:
28875799
Ncbi Gene Id Number:
887
Background Reference 1:
Olszewska-Pazdrak B, Townsend CM Jr, Hellmich MR. Agonist-independent activation of Src tyrosine kinase by a cholecystokinin-2 (CCK2) receptor splice variant. J Biol Chem. 2004 Sep 24;279(39):40400-4. Epub 2004 Jul 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-378

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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