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Western blot analysis of lysates from Recombinant Mouse CCL11 / Eotaxin Protein using CCL11 / Eotaxin Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 10ng,5ng,1ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.
Western blot analysis of lysates from Recombinant Mouse CCL11 / Eotaxin Protein using CCL11 / Eotaxin Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 10ng,5ng,1ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.

CCL11 / Eotaxin Rabbit Recombinant Antibody (83-280)

CATALOG NUMBER: 83-280

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:4000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Recombinant Mouse CCL11 / Eotaxin Protein (RP01635)
Predicted Molecular Weight:
11kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CCL11
Additional Names:
Scya11; eotaxin
Protein Accession Number:
P48298
Ncbi Gene Id Number:
20292
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to enable CCR3 chemokine receptor binding activity; chemokine activity; and protein dimerization activity. Involved in several processes, including modulation of age-related behavioral decline; negative regulation of neurogenesis; and positive regulation of angiogenesis. Acts upstream of or within branching involved in mammary gland duct morphogenesis and mammary duct terminal end bud growth. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in embryo; endocardial lining; liver parenchyma; liver vasculature; and yolk sac blood vessel. Human ortholog(s) of this gene implicated in asthma; human immunodeficiency virus infectious disease; hyperglycemia; and multiple sclerosis. Orthologous to several human genes including CCL11 (C-C motif chemokine ligand 11).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-280

List Size:
100 uL

List Price:

$477.00

Datasheet
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