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42-213 (6ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-213 (6ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CCL20 Antibody

CATALOG NUMBER: 42-213

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow, Pig
Immunogen:
The immunogen for this antibody is: CLGYTDRILHPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Human Peripheral Blood Mononucleocytes lysates gave no specific signal but low background (at antibody concentration up to 2ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Liver shows local staining between the hepatocytes.. Recommended concentration, 6-8ug/ml.
Specificity:
This antibody is expected to recognize both isoforms (NP_004582.1; NP_001123518.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CCL20
Additional Names:
CCL20, chemokine (C-C motif) ligand 20, CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38, exodus-1, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A (Cys-Cys), member 20
Protein Accession Number:
NP_004582.1,NP_001123518.1
Protein Gi Number:
4759076
Ncbi Gene Id Number:
6364
Background Reference 1:
Punj V, Matta H, Schamus S, Yang T, Chang Y, Chaudhary PM, Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation. Blood 2009 May 113 (22): 5660-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-213

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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