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Western blot analysis of lysates from THP-1 cells using CCL4/MIP-1 beta Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. THP-1 cells were treated with LPS (1 μg/ml), PMA/TPA (200 nM) and Brefeldin A (300 ng/ml) for 8 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of lysates from THP-1 cells using CCL4/MIP-1 beta Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. THP-1 cells were treated with LPS (1 μg/ml), PMA/TPA (200 nM) and Brefeldin A (300 ng/ml) for 8 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

CCL4/MIP-1 beta Rabbit Recombinant Antibody (83-544)

CATALOG NUMBER: 83-544

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:7000 - 1:42000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
THP-1 cells were treated with PMA, LPS and BFA
Predicted Molecular Weight:
10kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CCL4
Additional Names:
ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
Protein Accession Number:
P13236
Ncbi Gene Id Number:
6351
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-544

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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