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Antibody used in IP on Mouse Brain at: 1:50.
Antibody used in WB on Human Jurkat 0.125 ug/ml.
Antibody used in IP on Human NT2 at: 1:50.
Antibody used in WB on human NT2 line at 1:500.
Antibody used in IP on Mouse Brain at: 1:50.

CCNB1 Antibody

CATALOG NUMBER: 27-300

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCNB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CCNB1 antibody can be used for detection of CCNB1 by ELISA at 1:312500. CCNB1 antibody can be used for detection of CCNB1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
48 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CCNB1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CCNB1
Additional Names:
CCNB1, CCNB
Protein Accession Number:
NP_114172
Protein Gi Number:
14327896
Ncbi Gene Id Number:
891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Background Reference 1:
Zhao, M., (2006) Exp Oncol 28 (1), 44-48.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-300

List Size:
100 ul

List Price:

$519.00

Datasheet
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