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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

CCNB1 Antibody

CATALOG NUMBER: 55-627

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-411 amino acids from the C-terminal region of human CCNB1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCNB1
Additional Names:
G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Protein Accession Number:
P14635
Protein Gi Number:
116176
Ncbi Gene Id Number:
891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a regulatory proteininvolved in mitosis. The gene product complexes with p34(cdc2) toform the maturation-promoting factor (MPF). Two alternativetranscripts have been found, a constitutively expressed transcriptand a cell cycle-regulated transcript, that is expressedpredominantly during G2/M phase. The different transcripts resultfrom the use of alternate transcription initiation sites. [providedby RefSeq].
Background Reference 1:
Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
Background Reference 2:
van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
Background Reference 3:
Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
Background Reference 4:
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-627

List Size:
400 ul

List Price:

$495.00

Datasheet
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