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Western blot analysis of CCNB1IP1 Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CCNB1IP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CCNB1IP1 Antibody in NCI-H460 cell line lysates (35ug/lane)

CCNB1IP1 Antibody

CATALOG NUMBER: 63-926

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CCNB1IP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human CCNB1IP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCNB1IP1
Additional Names:
E3 ubiquitin-protein ligase CCNB1IP1, 632-, Cyclin-B1-interacting protein 1, Human enhancer of invasion 10, CCNB1IP1, C14orf18, HEI10
Protein Accession Number:
Q9NPC3
Protein Gi Number:
37078756
Ncbi Gene Id Number:
57820
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M.
Background Reference 1:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 2:
Gronholm, M., et al. Oncogene 25(32):4389-4398(2006)
Background Reference 3:
Toby, G.G., et al. Mol. Cell. Biol. 23(6):2109-2122(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-926

List Size:
400 ul

List Price:

$495.00

Datasheet
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