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Western blot analysis of CCNT1 in rat brain tissue lysate with CCNT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of CCNT1 in rat brain tissue with CCNT1 antibody at 2.5 μg/ml.
Immunofluorescence of CCNT1 in rat brain tissue with CCNT1 antibody at 20 μg/ml.
Western blot analysis of CCNT1 in rat brain tissue lysate with CCNT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CCNT1 Antibody

CATALOG NUMBER: 7223

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal CCNT1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CCNT1.
The immunogen is located within the last 50 amino acids of CCNT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CCNT1 antibody can be used for detection of CCNT1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 80 kDa
Purification:
CCNT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CCNT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CCNT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CCNT1
Additional Names:
CCNT1 Antibody: CCNT, CYCT1, HIVE1, Cyclin-T1, CycT1
Protein Accession Number:
NP_001231
Protein Gi Number:
17978466
Ncbi Gene Id Number:
904
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCNT1 Antibody: Cyclins function as regulators of CDK kinases and exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin-T1 protein (CCNT1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNT1 tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing CCNT1 and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. CCNT1 and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.
Background Reference 1:
Uhlmann F, Bouchoux C, and Lopez-Aviles S. A quantitative model for cyclin-dependent kinase control of the cell cycle: revisited. Philos. Trans. R. Soc. Lond. B. biol. Sci. 2011; 366:3572-83.
Background Reference 2:
Wei P, Garber ME, Fang SM, et al. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell 1998; 92:451-62.
Background Reference 3:
Mancebo HS, Lee G, Flygare J, et al. P-TEFb kinase is require for HIV Tat transcriptional activation in vivo and in vitro. Genes Dev. 1997 ; 11 :2633-44.
Background Reference 4:
Majello B and Napolitano G. Control of RNA polymerase II activity by dedicated CTD kinases and phosphatases. Front. Biosci. 2001; 6:D1358-68.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7223

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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