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Western blot analysis of extracts of various cell lines, using CCT3 antibody (22-303) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse heart using CCT3 antibody (22-303) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CCT3 antibody (22-303). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CCT3 antibody (22-303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

CCT3 Antibody

CATALOG NUMBER: 22-303

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCT3 (NP_005989.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
293T
Positive Control 3:
HepG2
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CCT3
Additional Names:
CCT3, chaperonin containing TCP1, subunit 3 (gamma), CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5, OTTHUMP00000025735, T-complex protein 1, gamma subunit, TCP1 (t-complex-1) ring complex, polypeptide 5, subunit 3
Ncbi Gene Id Number:
7203
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-303

List Size:
100 uL

List Price:

$436.00

Datasheet
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