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Antibody used in WB on Human 293T.
Antibody used in WB on Human HEK293T at: 1:1000 (Lane 1: Negative control (anti-FLAG antibody), Lane 2: Immunoprecipitation with CCT4 (C-term), Lane 3: 10 ug lysate).
Antibody used in IHC on Rat Hippocampal Neurons at 1:200.
Antibody used in WB on Human HEK 293T at 1:1000 dilution.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human 293T.

CCT4 Antibody

CATALOG NUMBER: 28-041

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCT4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CCT4 antibody can be used for detection of CCT4 by ELISA at 1:312500. CCT4 antibody can be used for detection of CCT4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
58 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CCT4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CCT4
Additional Names:
CCT4, SRB, Cctd, CCT-DELTA
Protein Accession Number:
NP_006421
Protein Gi Number:
38455427
Ncbi Gene Id Number:
10575
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCT4 is a subunit of a cytosolic hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55.
Background Reference 1:
Kimura, K., et al., (2006) Genome Res. 16 (1), 55-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-041

List Size:
100 ul

List Price:

$473.00

Datasheet
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