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Immunohistochemical analysis of paraffin - embedded human breast ductal myoepithelium showing cytoplasmic localization using CD10 antibody with DAB staining.
Immunohistochemical analysis of paraffin - embedded human lymph tissue showing membrane localization using CD10 antibody AEC staining.
Immunohistochemical analysis of paraffin - embedded human breast ductal myoepithelium showing cytoplasmic localization using CD10 antibody with DAB staining.

CD10 Antibody [2A1H5E1]

CATALOG NUMBER: 32-127

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human CD-10 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
IHC(P):Dilution 1:100 - 1:200
IHC(F):Dilution 1:200 - 1:400
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein G affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG2b
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CD10 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MME
Additional Names:
EPN, Atriopeptidase, CALLA, NEP, SFE, CD10
Protein Accession Number:
P08473
Protein Gi Number:
128062
Ncbi Gene Id Number:
4311
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD10 is a 100 kDa glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Background Reference 1:
Sato Y,et.al. Journal of Gastroenterology,1996 Feb.31(1):12-7.
Background Reference 2:
Inukai T,et.al. British Journal of Haematology,1995,89(3):623-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-127

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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