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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit mAb at 1:3000 dilution incubated overnight at 4°C. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | Exposure time: 60 s.
Western blot analysis of various lysates using CD10 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): NIH/3T3 | Exposure time: 20 s.
Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T cells (right) were surface-stained with CD10 Rabbit mAb or Rabbit IgG isotype control. followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control or CD10 Rabbit mAb, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit mAb at 1:3000 dilution incubated overnight at 4°C. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | Exposure time: 60 s.

CD10 Rabbit Recombinant Antibody (83-138)

CATALOG NUMBER: 83-138

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:1000 - 1:5000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293F transfected with CD10 (Mouse), Mouse kidney
Predicted Molecular Weight:
86kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NEP; SFE; CD10; CALLA; 6030454K05Rik
Additional Names:
Mme
Protein Accession Number:
Q61391
Ncbi Gene Id Number:
17380
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables peptidase activity. Involved in amyloid-beta clearance; positive regulation of long-term synaptic potentiation; and sensory perception of pain. Acts upstream of or within amyloid-beta metabolic process. Located in several cellular components, including dendrite; neuron projection terminus; and synaptic vesicle. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; liver and biliary system; and mammary gland. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in Alzheimer's disease; Charcot-Marie-Tooth disease axonal type 2T; cerebellar ataxia type 43; cerebral amyloid angiopathy; and membranous glomerulonephritis. Orthologous to human MME (membrane metalloendopeptidase).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-138

List Size:
100 uL

List Price:

$477.00

Datasheet
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