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Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549,K562 cell line lysates (35ug/lane).
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

CD11b Antibody

CATALOG NUMBER: 55-325

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:500
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
127 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ITGAM
Additional Names:
Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A
Protein Accession Number:
P11215
Protein Gi Number:
1708572
Ncbi Gene Id Number:
3684
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD11b is the integrin alpha M chain. Integrinsare heterodimeric integral membrane proteins composed of an alphachain and a beta chain. This I-domain containing alpha integrincombines with the beta 2 chain (ITGB2) to form a leukocyte-specificintegrin referred to as macrophage receptor 1 ('Mac-1'), orinactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2integrin is important in the adherence of neutrophils and monocytesto stimulated endothelium, and also in the phagocytosis ofcomplement coated particles.
Background Reference 1:
Gjelstrup, L.C., et al. J. Immunol. 185(7):4154-4168(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Warchol, T., et al. DNA Cell Biol. (2010) In press :
Background Reference 4:
Fan, Y., et al.Eur Acad Dermatol Venereol (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-325

List Size:
400 ul

List Price:

$495.00

Datasheet
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