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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SW480 whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody staining CD130 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + SW480 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SW480 whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 ug per lane.

CD130 Antibody

CATALOG NUMBER: 55-632

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 871-899 amino acids from the C-terminal region of human CD130.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:25
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL6ST
Additional Names:
Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, gp130, Oncostatin-M receptor subunit alpha, CD130, IL6ST
Protein Accession Number:
P40189
Protein Gi Number:
215273999
Ncbi Gene Id Number:
3572
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a signal transducershared by many cytokines, including interleukin 6 (IL6), ciliaryneurotrophic factor (CNTF), leukemia inhibitory factor (LIF), andoncostatin M (OSM). This protein functions as a part of thecytokine receptor complex. The activation of this protein isdependent upon the binding of cytokines to their receptors. vIL6, aprotein related to IL6 and encoded by the Kaposi sarcoma-associatedherpesvirus, can bypass the interleukin 6 receptor (IL6R) anddirectly activate this protein. Knockout studies in mice suggestthat this gene plays a critical role in regulating myocyteapoptosis. Alternatively spliced transcript variants encodingdistinct isoforms have been described. A related pseudogene hasbeen identified on chromosome 17.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Goette, N.P., et al. Exp. Hematol. 38(10):868-876(2010)
Background Reference 3:
Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) :
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-632

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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