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45-030 (1ug/ml) staining of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.
45-030 (4ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-030 (1ug/ml) staining of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.

CD14 Antibody

CATALOG NUMBER: 45-030

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KRVDADADPRQYAD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3ug/ml..An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD14
Additional Names:
CD14, CD14 antigen , HGNC:1628
Protein Accession Number:
NP_000582.1
Protein Gi Number:
4557417
Ncbi Gene Id Number:
929
Background Reference 1:
Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y. The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain. Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-030

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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