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SDS-PAGE Analysis of Purified, BSA-Free CD14 Antibody (clone LPSR/927). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free CD14 Antibody (clone LPSR/927). Confirmation of Integrity and Purity of the Antibody.

CD14 Antibody [LPSR/927]

CATALOG NUMBER: 33-302

Clonality:
Monoclonal
Tested Applications:
Flow, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Human CD14 recombinant protein was used as immunogen for this CD14 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
Immunofluorescence: 0.5-1 ug/ml


Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD14
Additional Names:
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Ncbi Gene Id Number:
929
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-302

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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