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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD161 using CD161 Rabbit mAb at 1:3000 dilution.  | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.5s.
Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with CD161 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD161 Rabbit mAb.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD161 using CD161 Rabbit mAb at 1:3000 dilution.  | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.5s.

CD161 Rabbit Recombinant Antibody (83-097)

CATALOG NUMBER: 83-097

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF-P, WB
Application Note:
WB,1:3000 - 1:18000, IF-P,1:200 - 1:2000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
25kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KLRB1
Additional Names:
NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A; KLRB1
Protein Accession Number:
Q12918
Ncbi Gene Id Number:
3820
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-097

List Size:
100 uL

List Price:

$477.00

Datasheet
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