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Western blot analysis of CD163 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD163 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of CD163 antibody in K562 cell line lysates (35ug/lane)

CD163 Antibody

CATALOG NUMBER: 62-830

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD163 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-274 amino acids from the N-terminal region of human CD163.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
125 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD163
Additional Names:
Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163, sCD163, CD163, M130
Protein Accession Number:
Q86VB7
Protein Gi Number:
313104083
Ncbi Gene Id Number:
9332
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
Background Reference 1:
Buehler,P.W., Abraham,B. Blood 113 (11), 2578-2586 (2009)
Background Reference 2:
Fabriek,B.O., van Bruggen,R. Blood 113 (4), 887-892 (2009)
Background Reference 3:
Strauss,M. and Levy,A.P. Mol. Cell. Biochem. 317 (1-2), 131-135 (2008)
Background Reference 4:
Groselj-Grenc,M., Ihan,A. Mediators Inflamm. 2008, 202646 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-830

List Size:
400 ul

List Price:

$495.00

Datasheet
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