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Western blot analysis of extracts of various cell lines, using CD168 antibody (14-913) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded human breast cancer using CD168 antibody (14-913) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using CD168 antibody (14-913) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using CD168 antibody (14-913) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

CD168 Antibody

CATALOG NUMBER: 14-913

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of mouse CD168
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse thymus
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 84-130kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HMMR
Additional Names:
AA386826, CD168, Rhamm, hyaluronan mediated motility receptor, intracellular hyaluronic acid-binding protein, receptor for hyaluronan-mediated motility
Ncbi Gene Id Number:
15366
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-913

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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