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<b> Flow-cytometry using the anti-CD18 antibody 1B4 ( </b> Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of 1B4 (24-652- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of human peripheral blood monocytes using anti-CD18 antibody (1B4 </b> Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of 1B4 (24-652) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-652- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-CD18 antibody 1B4 ( Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of 1B4 (24-652- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

CD18 Antibody [1B4], Rabbit IgG

CATALOG NUMBER: 24-652

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, Flow, IF, IP, RIA
Species Reactivity:
Human, Rhesus Monkey
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human, Rhesus Monkey
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IP, RIA
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
ITGB2
Additional Names:
Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta, Complement receptor C3 subunit beta, ITGB2, MFI7, IB4, m1B4, mIB4
Ncbi Gene Id Number:
3689
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-652

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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