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Western blot analysis of lysate from Ramos cell line, using CD19Antibody at 1:500.
Western blot analysis in Raji cell line lysates (35ug/lane).
Western blot analysis of CD19 using rabbit polyclonal CD19 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2).
Immunofluorescence analysis of CD19 Antibody with paraffin-embedded human lymph tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from Ramos cell line, using CD19Antibody at 1:500.

CD19 Antibody

CATALOG NUMBER: 58-120

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:500
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD19
Additional Names:
B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19
Protein Accession Number:
P15391
Protein Gi Number:
160332376
Ncbi Gene Id Number:
930
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Background Reference 1:
Deaglio,S., Blood 109 (12), 5390-5398 (2007)
Background Reference 2:
Bradbury,L.E., J. Immunol. 149 (9), 2841-2850 (1992)
Background Reference 3:
Kozmik,Z., Mol. Cell. Biol. 12 (6), 2662-2672 (1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-120

List Size:
400 ul

List Price:

$519.00

Datasheet
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