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Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.

CD227/MUC1 (phospho Tyr1229) Antibody

CATALOG NUMBER: 79-825

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.
Predicted Molecular Weight:
170 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MUC1
Additional Names:
PUM, EMA, H23AG, MUC-1, PEM
Protein Accession Number:
NP_001018016.1
Protein Gi Number:
324120958
Ncbi Gene Id Number:
4582
Background:
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Background Reference 1:
Lan M.S., J. Biol. Chem. 265:15294-15299(1990).
Background Reference 2:
Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
Background Reference 3:
Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-825

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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