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42-220 (2ug/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir Willi
42-220 (2ug/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir Willi

CD244 Antibody

CATALOG NUMBER: 42-220

Clonality:
Polyclonal
Tested Applications:
ELISA, IP
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EFLTIpYEDVKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 75kDa band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1kDa according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected. Recommended concentration: 1-3ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD244
Additional Names:
CD244, CD244 molecule, natural killer cell receptor 2B4, 2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 natural killer cell receptor 2B4, NK cell activation inducing ligand NAIL, natural killer cell receptor 2B4
Protein Accession Number:
NP_057466.1
Protein Gi Number:
7706529
Ncbi Gene Id Number:
51744
Background Reference 1:
Tangye SG, Lazetic S, Woollatt E, Sutherland GR, Lanier LL, Phillips JH Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP. J Immunol. 1999 Jun 15;162(12):6981-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-220

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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