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Western blot analysis of various lysates, using CD26/DPP4 Rabbit mAb at 1:1500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Imunoprecipitation of CD26/DPP4 from 300 µg extracts of Hep G2 cells treated by 1%Formaldehyde(10min) and Glycine (5min) was performed using 2 µg of CD26/DPP4 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using CD26/DPP4 Rabbit mAb at a dilution of 1:1000.
Flow cytometry: 1X10^6 K-562 cells (negative control,left) and NCI-H2452 cells (right) were surface-stained with CD26/DPP4 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 NCI-H2452 cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD26/DPP4 Rabbit mAb.
Western blot analysis of various lysates, using CD26/DPP4 Rabbit mAb at 1:1500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

CD26/DPP4 Rabbit Recombinant Antibody (82-564)

CATALOG NUMBER: 82-564

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IP, WB
Application Note:
WB,1:1000 - 1:5000, IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells, FC,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HepG2
Predicted Molecular Weight:
88kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DPP4
Additional Names:
CD26; ADABP; ADCP2; DPPIV; TP103; CD26/DPP4
Protein Accession Number:
P27487
Ncbi Gene Id Number:
1803
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-564

List Size:
100 uL

List Price:

$477.00

Datasheet
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