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Western blot analysis in Ramos cell line lysates (35ug/lane).
Western blot analysis of CD27 using rabbit polyclonal CD27 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD27 gene.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Ramos cell line lysates (35ug/lane).

CD27 Antibody

CATALOG NUMBER: 57-238

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the C-terminal region of human CD27.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD27
Additional Names:
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, CD27, TNFRSF7
Protein Accession Number:
P26842
Protein Gi Number:
269849546
Ncbi Gene Id Number:
939
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of theTNF-receptor superfamily. This receptor is required for generationand long-term maintenance of T cell immunity. It binds to ligandCD70, and plays a key role in regulating B-cell activation andimmunoglobulin synthesis. This receptor transduces signals thatlead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteinsTRAF2 and TRAF5 have been shown to mediate the signaling process ofthis receptor. CD27-binding protein (SIVA), a proapoptotic protein,can bind to this receptor and is thought to play an important rolein the apoptosis induced by this receptor.
Background Reference 1:
Jiang, J., et al. J. Clin. Immunol. 30(4):566-573(2010)
Background Reference 2:
Arimoto-Miyamoto, K., et al. Immunology 130(1):137-149(2010)
Background Reference 3:
Mizuochi, T., et al. J. Interferon Cytokine Res. 30(4):243-252(2010)
Background Reference 4:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-238

List Size:
400 ul

List Price:

$495.00

Datasheet
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