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Human CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD27, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human CD27 Ligand, Fc Tag with a linear range of 0.2-4 ng/mL (QC tested).
Human CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

CD27 / TNFRSF7 Recombinant Protein

CATALOG NUMBER: 11-501

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Thr 21 - Ile 192
Fusion Tag:
Mouse IgG Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.2 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
46.2 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Names:
CD27,TNFRSF7,S152,T14,Tp55
Protein Accession Number:
NP_001233.1
Background:
CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
Background Reference 1:
Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.
Background Reference 2:
Gravestein L.A., et al., 1998, Eur. J. Immunol. 28:2208-2216.
Background Reference 3:
Prasad K.V.S., et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94:6346-6351.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-501

List Size:
0.1 mg

List Price:

$577.00

Datasheet
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