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Western blot analysis of lysates from Mouse ovary using CD271 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.
Western blot analysis of lysates from Rat uterus using CD271 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90 s.
Flow cytometry: 1X10^6 293F cells (negative control,Left) and 293F (Transfection) cells (Right) were surface-stained with CD271/NGFR Rabbit mAb or Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with Rabbit IgG isotype control or CD271/NGFR Rabbit mAb, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
Western blot analysis of lysates from Mouse ovary using CD271 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.

CD271 Rabbit Recombinant Antibody (82-782)

CATALOG NUMBER: 82-782

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:1000 - 1:4000, FC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse ovary
Predicted Molecular Weight:
45KDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Ngfr
Additional Names:
p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16; CD271
Protein Accession Number:
Q9Z0W1
Ncbi Gene Id Number:
18053
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables death receptor activity; nerve growth factor binding activity; and small GTPase binding activity. Involved in several processes, including cellular glucose homeostasis; circadian regulation of gene expression; and dorsal aorta development. Acts upstream of or within several processes, including hair follicle morphogenesis; nervous system development; and regulation of signal transduction. Located in several cellular components, including cell surface; cell-cell junction; and postsynaptic density. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease and mental depression. Orthologous to human NGFR (nerve growth factor receptor).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-782

List Size:
100 uL

List Price:

$477.00

Datasheet
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