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Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).
Flow cytometry testing of paraformaldehyde-fixed human Jurkat cells with CD28 antibody (clone 204.12); Red= isotype control, Blue= CD28 antibody.
SDS-PAGE analysis of purified, BSA-free CD28 antibody (clone 204.12) as confirmation of integrity and purity.
Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).

CD28 Antibody [204.12]

CATALOG NUMBER: 33-494

Clonality:
Monoclonal
Tested Applications:
Flow, Functional Assays, IF
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Mouse, Pig, Sheep
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Mouse, Pig, Sheep
Immunogen:
Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, Functional Assays, IF
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Functional Activity (Order SAF formulation)
Optimal dilution of the CD28 antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD28
Additional Names:
CD4 Antibody [9H5A8] : CD4mut
Ncbi Gene Id Number:
920
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-494

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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