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42-448 (0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-448 (0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cd28 Antibody

CATALOG NUMBER: 42-448

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: KEKHLCHTQSSPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in Mouse Thymus lysates (calculated MW of 25.2kDa according to NP_031668.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Specificity:
The immunizing peptide represents a part of the extracellular domain.
Positive Control 1:
Cat. No. 1409 - Mouse Thymus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cd28
Additional Names:
Cd28, CD28 antigen, CH29-189K17.3, OTTMUSP00000002157, T-cell-specific surface glycoprotein CD28
Protein Accession Number:
NP_031668.3
Protein Gi Number:
161484597
Ncbi Gene Id Number:
12487(mouse);

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-448

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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