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Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of CD28 in human spleen tissue with CD28 antibody at 2 μg/ml.
Immunofluorescence of CD28 in human spleen tissue with CD28 antibody at 20 μg/ml.
Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CD28 Antibody

CATALOG NUMBER: 8683

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (94%), Cow: (94%), Cat: (94%), Rat: (89%), Mouse: (89%)
Immunogen:
CD28 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human CD28.
The immunogen is located within the last 50 amino acids of CD28.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
CD28 antibody can be used for Western blot at 1μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 28 kDa
Observed: 26 kDa
Purification:
CD28 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CD28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CD28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD28
Additional Names:
CD28 Antibody: CD28 Molecule, CD28 Antigen, Tp44, T-Cell-Specific Surface Glycoprotein CD28, T-Cell-Specific Surface Glycoprotein, CD28 Antigen (Tp44)
Protein Accession Number:
P10747
Ncbi Gene Id Number:
940
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8683

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

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