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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
CD2BP2 antibodyimmunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD2BP2 antibodyfor immunohistochemistry.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

CD2BP2 Antibody

CATALOG NUMBER: 55-522

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CD2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human CD2BP2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD2BP2
Additional Names:
CD2 antigen cytoplasmic tail-binding protein 2, CD2 cytoplasmic domain-binding protein 2, CD2 tail-binding protein 2, U5 snRNP 52K protein, U5-52K, CD2BP2, KIAA1178
Protein Accession Number:
O95400
Ncbi Gene Id Number:
10421
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a bi-functional protein. In thecytoplasm, the encoded protein binds the cytoplasmic tail of humansurface antigen CD2 via its C-terminal GYF domain, and regulateCD2-triggered T lymphocyte activation. In the nucleus, this proteinis a component of the U5 small nuclear ribonucleoprotein complexand is involved in RNA splicing. A pseudogene has been identifiedon chromosome 7. Alternative splicing results in multipletranscript variants but their biological validity has not beendetermined.
Background Reference 1:
Heinze, M., et al. Int. Immunol. 19(11):1313-1318(2007)
Background Reference 2:
Nielsen, T.K., et al. J. Mol. Biol. 369(4):902-908(2007)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-522

List Size:
400 ul

List Price:

$495.00

Datasheet
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