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CD3 Antibody [Hit3a]

CATALOG NUMBER: 76-213

Clonality:
Monoclonal
Tested Applications:
Flow, Functional Assays, IHC, IP
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Flow, Functional Assays, IHC, IP
Specificity:
The Hit3a monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex.
Purification:
The monoclonal antibody was purified utilizing affinity chromatography.
Clonality:
Monoclonal
Isotype:
Mouse IgG2a, kappa
physical-state:
Liquid
Buffer:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Storage Conditions:
The product should be stored undiluted at 4°C . Do not freeze.
Ncbi Official Symbol:
CD3E
Additional Names:
T3E, TCRE, IMD18, CD3E
Ncbi Gene Id Number:
916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Hit3a monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction.The HIT3a antibody cross-links with the TCR complex, initiating the cellular activation and proliferation, but it cannot be used for intracellular CD3 staining, as it is able to stain only the surface CD3 complex.
Background Reference 1:
McMichael, A. J. (1987). Leucocyte typing III.Oxford University Press, Oxford. Norton AJ, Isaacson PG (1985)
Background Reference 2:
Knapp W(1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.
Background Reference 3:
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York
Background Reference 4:
Lanier, L. L., Allison, J. P., Phillips, J. H. (1986). Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation.The Journal of Immunology,137(8), 2501-2507.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

76-213

List Size:
0.1 mg

List Price:

$217.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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