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43-634 (0.1ug/ml) staining of Jurkat (A) and (0.3ug/ml) MOLT4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-634 (0.1ug/ml) staining of Jurkat (A) and (0.3ug/ml) MOLT4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CD3-ZETA / CD247 Antibody

CATALOG NUMBER: 43-634

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KNPQEGLYNELQKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16-17kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6kDa according to NP_000725.1). Recommended concentration: 0.1-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_932170.1; NP_000725.1).
Predicted Molecular Weight:
Approx 16 + 17kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD3-ZETA / CD247
Additional Names:
CD247, CD247 molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, TCR zeta chain
Protein Accession Number:
NP_932170.1,NP_000725.1
Protein Gi Number:
23830999
Ncbi Gene Id Number:
919
Background Reference 1:
Teruel M, McKinney C, Balsa A, Pascual-Salcedo D, Rodriguez-Rodriguez L, Ortiz AM, Gómez-Vaquero C, González-Gay MA, Smith M, Witte T, Merriman T, Lie BA, Martin J.Association of CD247 polymorphisms with rheumatoid arthritis: a replication study and a meta-analysis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-634

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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