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Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 ζ (Phospho-Tyr142) Antibody. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 ζ (Phospho-Tyr142) Antibody. The lane on the left is treated with antigen-specific peptide.

CD3 zeta (phospho Tyr142) Antibody

CATALOG NUMBER: 79-785

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
CD3 zeta (phospho Tyr142) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 142 (G-L-Y (p)-Q-G) derived from Human CD3 ζ.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142.
Predicted Molecular Weight:
20 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CD247
Additional Names:
T3Z, CD3-zeta, TCRZ, T-cell receptor T3 zeta chain
Protein Accession Number:
NP_000725.1
Protein Gi Number:
23830999
Ncbi Gene Id Number:
919
Background:
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Background Reference 1:
Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029.
Background Reference 2:
Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423.
Background Reference 3:
Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330.
Background Reference 4:
Hisashi Arase, J. Immunol., Jan 2001; 166: 21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-785

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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