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Human CD30 Ligand, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human CD30, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD30 Ligand, His Tag, low endotoxin with a linear range of 0.039-0.313 ug/mL (QC tested).
Immobilized Human CD30 Ligand, His Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin with a linear range of 0.6-10 ng/mL (Routinely tested).
Human CD30 Ligand, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

CD30 Ligand / TNFSF8 Recombinant Protein

CATALOG NUMBER: 11-332

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln 63 - Asp 234
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
21.5 kDa
Purity:
>90% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TNFSF13
Additional Names:
CD30-L,CD153,TNFSF8,CD30L,CD30LG,CD153 antigen,CD30 antigen ligand,CD30 Ligand
Protein Accession Number:
NP_003799.1
Ncbi Gene Id Number:
8741
Background:
CD30 ligand (CD30L) and CD30 are interacting cellsurface glycoproteins that are members of the tumour necrosis factor (TNF) and tumour necrosis factor receptor(TNFR) superfamilies. In humans, CD30L is expressed at high levels on activated T cells. There is conflicting evidence with regard to cell surface expression of CD30L on primary human B cells. An initial report of constitutive CD30L expression on peripheral blood B cells33 has not been substantiated.
Background Reference 1:
Marín ND., et al. 2017, Tuberculosis (Edinb)., 102, 8-15.
Background Reference 2:
Kennedy MK., et al. 2006, Immunology., 118(2), 143-52.
Background Reference 3:
Horie R., et al. 1998, Semin Immunol., 10(6), 457-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-332

List Size:
0.1 mg

List Price:

$577.00

Datasheet
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