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Western blot analysis of various lysates, using CD305/LAIR1 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Flow cytometry: 1X10^6 K-562 cells(negative control,left) and THP-1 cells (right) were surface-stained with PSFlo 647 Rabbit anti-CD305/LAIR1 Rabbit mAb(2 μg/mL,orange line)or PSFlo 647 Rabbit IgG isotype control.Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 THP-1 cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD305/LAIR1 Rabbit mAb(2 μg/mL,right).
Western blot analysis of various lysates, using CD305/LAIR1 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

CD305/LAIR1 Rabbit Recombinant Antibody (82-736)

CATALOG NUMBER: 82-736

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:1000 - 1:5000, FC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
THP-1
Predicted Molecular Weight:
23kDa/29kDa/31kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
hLAIR1
Additional Names:
LAIR1; CD305; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1; CD305/LAIR1
Protein Accession Number:
Q6GTX8
Ncbi Gene Id Number:
3903
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-736

List Size:
100 uL

List Price:

$477.00

Datasheet
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