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Western blot analysis of extracts of various cell lines, using CD31 antibody (14-042) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver cancer using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using CD31 antibody (14-042) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

CD31 Antibody

CATALOG NUMBER: 14-042

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31 (NP_001292086.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
THP-1
Positive Control 2:
Mouse lung
Positive Control 3:
Mouse spleen
Positive Control 4:
Mouse heart
Predicted Molecular Weight:
Observed: 130kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PECAM1
Additional Names:
Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam, Pecam-1
Ncbi Gene Id Number:
18613
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-042

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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