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<b> Immunofluorescence staining of fixed U937 cells with anti-CD33 antibody M195 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of M195 (31-536) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 31-536- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Immunofluorescence staining of fixed U937 cells with anti-CD33 antibody M195 ( Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of M195 (31-536) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 31-536- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

CD33 Antibody [M195], Rabbit IgG

CATALOG NUMBER: 31-536

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, RIA, Therapeutics
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
The original antibody was generated by immunizing mice with live leukemic myeloblasts.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, RIA, Therapeutics
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
CD33
Additional Names:
gp67, Siglec-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3
Ncbi Gene Id Number:
945
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

31-536

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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