CD34 Antibody [4H5E7 / 9B10D4]

CATALOG NUMBER: 32-132

Clonality:
Monoclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant CD34 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Dilution
Western Blot:1:200 - 1:1,000
IF:1:25 - 1:100
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CD34 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD34
Additional Names:
Hematopoietic progenitor cell antigen CD34, CD_antigen=CD34
Protein Accession Number:
P28906
Protein Gi Number:
3183511
Ncbi Gene Id Number:
947
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Background Reference 1:
Sho E., et al. Arterioscler Thromb Vasc Biol. 2004: 24: 1916-21.
Background Reference 2:
Satterthwaite, A.B.,et al. Genomics.1992:12:788-794.
Background Reference 3:
Krause, D.S.,et al. 1994. Blood.84: 691-701.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-132

List Size:
0.1 mL

List Price:

$469.00

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