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43-633 (1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-633 (1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CD3D isoform A Antibody

CATALOG NUMBER: 43-633

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HETGRLSGAADTQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 20kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 18.9kDa according to NP_000723.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize reported isoform A (NP_000723.1) only.
Predicted Molecular Weight:
Approx 20kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD3D
Additional Names:
CD3D, CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, IMD19, T3D, CD3 antigen, delta subunit, CD3 delta, CD3d antigen, delta polypeptide (TiT3 complex), OKT3, delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain
Protein Accession Number:
NP_000723.1
Protein Gi Number:
4502669
Ncbi Gene Id Number:
915
Background Reference 1:
Gil J, Busto EM, Garcillán B, Chean C, García-Rodríguez MC, Díaz-Alderete A, Navarro J, Reiné J, Mencía A, Gurbindo D, Beléndez C, Gordillo I, Duchniewicz M, Höhne K, García-Sánchez F, Fernández-Cruz E, López-Granados E, Schamel WW, Moreno-Pelayo MA, ReciA leaky mutation in CD3D differentially affects αβ and γδ T cells and leads to a Tαβ-Tγδ+B+NK+ human SCID.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-633

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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