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Western blot testing of human Jurkat lysate in the absence and presence of immunizing peptide, and mouse thymus lysate probed with CD3e antibody.
Western blot testing of Jurkat lysate with CD3e antibody.
Western blot testing of human Jurkat lysate in the absence and presence of immunizing peptide, and mouse thymus lysate probed with CD3e antibody.

CD3e Antibody

CATALOG NUMBER: 33-082

Clonality:
Polyclonal
Tested Applications:
Flow, IP, WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Cat, Chicken, Dog, Elephant, Frog, Guinea Pig, Hamster, Horse, Human, Mole Rat, Mouse, Opossum, Pig, Primate, Rabbit, Rat, Sheep
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Cat, Chicken, Dog, Elephant, Frog, Guinea Pig, Hamster, Horse, Human, Mole Rat, Mouse, Opossum, Pig, Primate, Rabbit, Rat, Sheep
Immunogen:
Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IP, WB
Application Note:
Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cells
WB: 0.5-1.0 ug/ml
IP: 1-2ug/500ug protein lysate

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.
Purification:
Protein A purified CD3e antibody
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD3E
Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Ncbi Gene Id Number:
916
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-082

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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