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43-627 (0.1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-627 (0.1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CD3E Antibody

CATALOG NUMBER: 43-627

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig
Immunogen:
The immunogen for this antibody is: C-RKGQRDLYSGLNQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1). Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificity:
This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.
Predicted Molecular Weight:
Approx 22kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD3E
Additional Names:
CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain
Protein Accession Number:
NP_000724.1
Protein Gi Number:
1345708
Ncbi Gene Id Number:
916
Background Reference 1:
Gagnon E, Schubert DA, Gordo S, Chu HH, Wucherpfennig KW.Local changes in lipid environment of TCR microclusters regulate membrane binding by the CD3ε cytoplasmic domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-627

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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