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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis of CD3E using rabbit polyclonal CD3E Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD3E gene.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

CD3E Antibody

CATALOG NUMBER: 57-098

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This CD3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the C-terminal region of human CD3E.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD3E
Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Protein Accession Number:
P07766
Protein Gi Number:
1345708
Ncbi Gene Id Number:
916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, andthe T-cell receptor alpha/beta and gamma/delta heterodimers, formsthe T-cell receptor-CD3 complex. This complex plays an importantrole in coupling antigen recognition to several intracellularsignal-transduction pathways. The genes encoding the epsilon, gammaand delta polypeptides are located in the same cluster onchromosome 11. The epsilon polypeptide plays an essential role inT-cell development. Defects in this gene cause immunodeficiency.This gene has also been linked to a susceptibility to type Idiabetes in women.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 3:
Brophy, K., et al. BMC Med. Genet. 11, 76 (2010) :
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-098

List Size:
400 ul

List Price:

$495.00

Datasheet
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